Collisions of electrons with atoms and molecules that drive chemical and physical reactions are of utmost importance for a broad range of areas from plasma physics to radiation damage in living tissue or the chemistry in planetary atmospheres. Moreover, they are of basic intrinsic interest since they constitute one of the most fundamental realizations of the ubiquitous correlated quantum-dynamical few-body problem. In this talk, I will present our recent results of electron impact ionization from simple atoms to complex bio-molecule targets. Kinematically complete experiments for electron impact ionization will be discussed for studies of simple targets, which determine the momentum vectors of all continuum particles for known final-state configuration of the remaining ion, thus ultimately benchmarking all facets of quantum few-body Coulomb dynamics. Electron impact ionization of tetrahydrofuran, as a typical bio-molecule target, was recently studied using our reaction microscope. I will present the recent results about low-energy (E0 = 26 eV) electron-impact ionization and fragmentation of the tetrahydrofuran molecule. The momenta of all three final state charged particles are measured in triple coincidence. The fragment products are identified in the measured mass spectrum. A detailed analysis of the reaction processes will be presented.